PSL Spheres 20nm to 1um, NIST Traceability Size Accuracy

PSL Spheres, 20 – 900nm, Polystyrene Latex Spheres, 3000 Series

3000 Series PSL are excellent for use with any application that requires a NIST traceable size standard with a very narrow standard deviation. These PSL Size Standards are highly uniform polystyrene spheres calibrated within nanometers with NIST traceable methodology. One nanometer is 0.001 µm or 10 Angstroms. Nanosphere size standards are ideal for the calibration of electron and atomic force microscopes, as well as size calibration or wafer inspection systems, also referred to as SSIS or Surface Scanning Inspection Systems. They are also used in laser light scattering studies and colloidal systems research. The 20 to 900 nm range of spheres is convenient for checking the sizes of bacteria, viruses, ribosomes and sub-cellular components.

The Polystyrene latex spheres are available as uniform polymer spheres in a range of discrete sizes from 20 to 900 nanometers (nm). The spherical diameters are calibrated with linear dimensions transferred from NIST. Spheres are used instead of irregularly shaped particles to minimize the response of laser scanners which are sensitive to shaped particles. The standards are packaged as aqueous suspensions in 15 milliliter (mL) dropper-tipped bottles. The particle concentrations are optimized for ease of dispersion and colloidal stability. The spheres have a density of 1.05 g/cm3 and an index of refraction of 1.59 @ 589 nm, measured at 25 degrees centigrade.

Each package contains a Certificate of Calibration and Traceability to NIST which includes a description of the calibration method and its uncertainty, and a table of chemical and physical properties. A Material Safety Data Sheet, with handling and disposal instructions, is also available. PSL bottles are lot-numbered for convenient technical service and support after the sale.

 

PSL Spheres 20nm to 900 nm
Particle Composition Polystyrene Latex, PSL Spheres
Particle Density 1.05 g/cm³
Index of Refraction 1.59 @ 589nm (25°C)
Bottle Size 15 mL
Expiration Date ≤ 24 months
Additives Contains trace amounts of surfactant
Suggested Storage Temp. 2-8°C
Bottle Size and Volume 15ml (A) Bottle

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PSL Spheres, 20nm to 900nm, 3000 Series

Product Part #

Nominal Diameter

Certified Mean Peak

Std. Dev & CV

Solids Content

AP3020A   

 20 nm

21 nm ± 1 nm

Not determined

1%

AP3030A   

 30 nm

30 nm ± 1 nm

Not determined

1%

AP3040A   

 40 nm

40 nm ± 1 nm

Not determined

1%

AP3050A   

 50 nm

46 nm ± 2 nm

7.3 nm (15.8%)

1%

AP3060A   

 60 nm

59 nm ± 2 nm

9.9 nm (16.8%)

1%

AP3070A   

 70 nm

73 nm ± 2 nm

5.7 nm (7.8%)

1%

AP3080A   

 80 nm

81 nm ± 3 nm

5.8 nm (7.2%)

1%

AP3090A   

 90 nm

92 nm ± 3 nm

7.0 nm (7.6%)

1%

AP3100A   

 100 nm

97 nm ± 3 nm

4.9 nm (5.0%)

1%

AP3125A   

 125 nm

125 nm ± 3 nm

5.4 nm (4.3%)

1%

AP3150A   

 150 nm

151 nm ± 4 nm

5.1 nm (3.4%)

1%

AP3200A   

 200 nm

200 nm ± 4 nm

3.4 nm (1.7%)

1%

AP3220A   

 220 nm

220 nm ± 4 nm

4.6 nm (2.1%)

1%

AP3240A   

 240 nm

240 nm ± 5 nm

3.7 nm (1.5%)

1%

AP3269A   

 270 nm

269 nm ± 5 nm

4.2 nm (1.6%)

1%

AP3300A   

 300 nm

300 nm ± 6 nm

5.1 nm (1.7%)

1%

AP3350A   

 350 nm

350 nm ± 6 nm

5.3 nm (1.5%)

1%

AP3400A   

 400 nm

400 nm ± 9 nm

7.3 nm (1.8%)

1%

AP3450A   

 450 nm

453 nm ± 9 nm

7.9 nm (1.7%)

1%

AP3495A   

 500 nm

491 nm ± 4 nm

6.3 nm (1.3%)

1%

AP3500A   

 500 nm

498 nm ± 9 nm

7.9 nm (1.6%)

1%

AP3560A   

 560 nm

565 nm ± 9 nm

10.0 nm (1.8%)

1%

AP3600A   

 600 nm

596 nm ± 9 nm

12.2 nm (2.0%)

1%

AP3700A   

 700 nm

707 nm ± 9 nm

8.3 nm (1.2%)

1%

AP3800A   

 800 nm

799 nm ± 9 nm

4.8 nm (0.6%)

1%

AP3900A   

 900 nm

903 nm ± 12 nm

4.1 nm (0.5%)

1%

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